Pigeonpea

1. The Plant Variety Protection (PVP) Authority decides when, where and in what quantity and quality the plant material required for testing the variety is to be delivered. Applicants submitting material from a country other than India must make sure that all customs formalities are complied with. The minimum quantity of seed to be supplied by the applicant should be:

2000 grams (in one submission only)

2. The seed should meet the minimum requirements for germination capacity, moisture content and physical purity prescribed for certified seed in India . Especially for storage, which requires a higher standard, the applicant should state, the actual germination capacity which should be as high as possible. The seed supplied should be visibly healthy, not lacking in vigour or affected by any important pest or disease.

2. The plant material must not have undergone any treatment unless the competent authorities allow or request such treatment. If it has been treated, full details of the treatment must be given.

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III. Conduct of tests

1. The minimum duration of tests should normally be two independent similar growing seasons with reference to the ecosystem of the variety submitted for DUS test.

2. The test should normally be conducted at two test locations. If any important characteristics of the variety can not be seen at these places, the variety may be tested at an additional place.

3. The test should be carried out under conditions ensuring normal growth. The size of the plot should be such that plants or parts of plant may be removed for measuring and counting without prejudice to the observation which must be made up to the end of the growing period. Each test should include a minimum of 300 plants which should be divided among 3 replications. Separate plots for observation and for measuring can only be used if they have been subjected to similar environmental conditions.

Test plot design

No. of rows	: 6
Row length	: 5m
Row to row distance	: 60cm(early varieties,80cm(late varieties)
Plant to plant distance	: 20cm
Number of replications	: 3

4. Observations should not be recorded on plants in border rows.

5. Additional tests for special purpose may be established.

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IV. Methods and observations

1. The characteristics described in the Table of characteristics should be used for the testing of varieties for DUS

2. For the assessment of distinctness and stability, observations should be made on 30 plants or parts of 30 plants which should be divided among 3 replications (10 plants per replication).

3. For the assessment of uniformity of characteristics on the plot as a whole (visual assessment by a single observation of a group of plants or parts of plants), a population standard of 1% with an acceptance probability of at least 95% should be applied. In the case of a sample size of 300 plants, the number of off-types should not exceed 4.

4. All observations on growth habit and leaf should be made at the time of full flowering unless indicated otherwise.

5. All observations on the pod should be made at dough stage unless indicated otherwise.

6. All observations on the seed should be made on harvested dry seeds.

7. For the assessment of colour characteristics, it is recommended that Royal Horticultural Society (RHS) colour chart should be used.

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V. Grouping of varieties

1. The collection of varieties to be grown should be divided into groups to facilitate the assessment of distinctness. Characteristics, which are suitable for grouping purposes, are those, which are known from experience not to vary, or to vary only slightly, within a variety. Their various states of expression should be fairly evenly distributed throughout the collection.

2. It is recommended that the competent authorities use the following characteristics for grouping varieties:

i. Time of flowering (characteristic 3)

ii. Plant: growth habit (characteristic 4 )

iii. Stem: colour (characteristic 5)

iv. Pod: waxiness (characteristic 12)

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VI. Characteristics and symbols

1. To assess distinctness, uniformity and stability, the characteristics and their states as given in the Table of characteristics should be used.

2. Notes (1 to 9), for the purposes of electronic data processing, are given opposite the states of each characteristic.

3. Legend

(*) Characteristics that should be used every growing period for the examinations of all varieties and should always be included in the description of the variety, except when the state of expression of a preceding characteristic or regional environmental conditions render this impossible.

(+) See explanations on the Table of characteristics in Chapter VII.

4. The optimum stage of plant growth for assessment of each characteristic is indicated in the sixth column of Table of characteristics.

5. Type of assessment of characteristics indicated in column seven of Table of characteristics is as follows :

MG : Measurement by a single observation of a group of plants or parts of plants

MS : Measurement of a number of individual plants or parts of plants

VG : Visual assessment by a single observation of a group of plants or parts of plants

VS : Visual assessment by observation of individual plants or parts of plants

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VII. Table of characteristics

S.No	Characteristics	States	Note	Example varieties	Stage of observation	Type of assessment
1. (*)	Plant: anthocyanin colouration of hypocotyl	absent present	1 9		seedling	VS
2. (+)	Plant: branching pattern	erect(<30⁰) semi-spreading(30⁰-60⁰⁾spreading(>60⁰)	3 5 7		insertion of first flower	MS
3. (*)	Time of flowering (50% of the plants with at least one open flower)	very early (<61 days) early(61-90 days) medium(91-130 days) late( 131-160 days) very late(>160 days)	1 3 5 7 9			VG
4. (*)	Plant: growth habit	indeterminate determinate	1 2		full flowering	VG
5. (*)	Stem : colour	green purple	1 2		full flowering	VG
6. (*) (+)	Leaf: shape	oblong obovate seasme	1 2 3		full flowering	VG
7. (*)	Leaf : pubescence on lower surface of the leaf	absent present	1 9		full flowering	VG
8. (*)	Flower: colour of petal	light yellow yellow orange yellow purple red	1 2 3 4 5		full flowering	VG
9. (*) (+)	Flower: pattern of streaks on petal	absent sparse medium dense mosaic	1 3 5 7 9		full flowering	VG
10. (*)	Pod: colour	green green with brown streaks green with purple streaks purple dark purple	1 2 3 4 5		premature pod(dough stage)	VG
11. (*)	Pod: pubescence	absent present	1 2		full podding	VG
12. (*)	Pod: waxiness	absent present	1 9		premature pods	VG
13.	Pod :surface stickiness	absent present	1 2		premature pods	VG
14. (*) (+)	Pod: constriction	slight prominent	3 7		premature pods	VG
15. (*)	Plant :height	short(<100 cm) medium(100-150cm) tall(>150 cm)	3 5 7		harvest maturity	MS
16. (*)	Seed :colour	cream brown dark brown grey purple	1 2 3 4 5		ripe seeds	VG
17. (*)	Seed :colour pattern	uniform mottled	1 2		ripe dry seeds	VG
18. (*)	Seed: shape	oval globular elongate	1 2 3		ripe seeds	VG
19.	Seed: size(100 seed weight )	small(<7g ) medium(7-8g ) large(9-11g ) very large(>11g)	3 5 7 9		ripe dry seeds	MG

VIII. Explanations on the table of characteristics


3 erect	5 semi-spreading	7 spreading

Characteristic 4. Plant : growth habit


1 indeterminate	2 determinate

Characteristic 6. Leaf : shape


1 oblong	2 obovate	3 seasme

Characteristic 9. Flower : pattern of streaks on petal


1 absent	2 sparse		3 medium

4 dense		5 mosaic

Characteristic 14. Pod : constriction


3 slight	7 prominent

Characteristic 17. Seed : colour pattern


1 uniform	2 mottled

Characteristic 18. Seed : shape


1 oval	2 globular	3 elongate

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IX. Technical questionnaire

				Reference Number (not to be filled in by the applicant

Technical questionnaire to be completed in connection with an application for plant breeders’ rights
1. Species (Cajanus cajan L.) Pigeonpea
2. Applicant (Name and address)
3. Proposed denomination or breeder’s reference
4. Information on origin, maintenance and reproduction of the variety 4.1 Genetic origin and breeding method 4.2 Other information
5. Characteristics of the variety to be indicated (the number in brackets refers to the corresponding characteristic in Test Guidelines; please mark the state of expression which best corresponds).
S.No	Characteristics		State		Example varieties		Note
1. (3)	Time of flowering (50% of the plants with at least one open flower)		very early (<61 days) early(61-90 days) medium(91-130 days) late( 131-160 days) very late(>160 days)				1[ ] 3[ ] 5[ ] 7[ ] 9[ ]
2. (4)	Plant: growth habit		indeterminate determinat				1[ ] 2[ ]
3. ( 5 )	Stem : colour		green purple				1[ ] 2[ ]
4. ( 12 )	Pod: waxiness		absent present				1[ ] 9[ ]
6. Similar varieties and differences from these varieties
Denomination of similar variety		Characteristic in which the similar variety is different @		State of expression of similar variety		State of expression of candidate variety

@ In the case of identical states of expressions of both varieties, please indicate the size of the difference.
7. Additional information which may help to distinguish the variety 7.1 Resistance to insects-pests and diseases 7.2 Special conditions for the examinatio n of the variety 7.3 Other information
8.3 Authorization for release (a) Does the variety require prior authorization for release under legislation concerning the protection of the environment, human and animal health? Yes { } No { } (b) Has such authorization been obtained? Yes { } No { }

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