INDIA

ITG/14

Date: 7-1-2004

National Guidelines

for the conduct of tests for

Distinctness, Uniformity and Stability

Jute

(Corchorus olitorius L./Corchorus capsularis L.)

 

Contents

i.    Subject
ii.   Materials required
iii.  Conduct of tests
iv.  Methods and observation
v.   Grouping of varieties
vi.  Characteristics and symbols
vii. Table of characteristics 
viii. Explanation on the table of characteristics
ix.  Technical questionnaire
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I.  Subject:  

                          These test guidelines apply to all varieties of Jute (Corchorus olitorius L  and  C. capsularis L.).

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II. Material required

  1. The Plant Variety Protection (PVP) Authority decides when, where and in what quantity and quality of the seed and the plant material required for testing the variety is to be delivered.  Applicants submitting material from a country other than India must make sure that all customs formalities are complied with.  The minimum quantity of seed to be supplied by the applicant should be:
      
     
    1000 grams      (in one submission only)
      
  2. The seed should meet the minimum requirements for germination capacity, moisture content and physical purity prescribed for certified seed in India. Especially for storage, which requires a higher standard, the applicant should state, the actual germination capacity  which should be as high as possible but not less than 85%. The seed supplied should be visibly healthy, not lacking in vigour or affected by any important pest or disease.
      
  3. The seed material must not have undergone any treatment unless the competent authority allow or request such treatment. If it has been treated, full details of the treatment must be given
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III. Conduct of tests

  1. The minimum duration of tests should normally be two independent growing seasons with reference to the ecosystem of the variety submitted for DUS test.
       
  2. The tests should normally be conducted at two test locations.  If any important characteristic of the variety cannot be seen at these places, the variety may be tested at an additional place.
      
  3. The field test should be carried out under conditions ensuring normal growth.  The size of the plots should be such that plants or parts of plants may be removed for measurement and counting without prejudice to the observations, which must be made up to the end of the growing period.  Each test should include a minimum of 500 plants which should be divided among three replications.  Separate plots for observation and for measurement can only be used if they have been subjected to similar environmental conditions.

    Test plot design  
    Number of rows: : 4
    Row length : 5 m
    Row to row distance : 40 cm
    Plant to plant distance : 5 to 7 cm
    Number of replications : 3

     

  4. Observations should not be recorded on plants in border rows.
      
  5. Additional tests for special purpose may be established.
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IV. Methods and observations

  1. The characteristics described in the Table of characteristics should be used for the testing of varieties and hybrids for DUS.
       
  2. For the assessment of distinctness and stability all observations should be made on at least 30 plants or parts of 30 plants which should be divided among three replications (10 plants in each replication).
       
  3. For the assessment of uniformity of characteristics on the plot as a whole (visual assessment by a single observation of a group of plants or parts of plants), the number of aberrant plants should not exceed 3 in 500.
       
  4. For the assessment of colour characteristics, it is recommended that Royal          Horticultural Society (RHS) colour chart should be used.
      
  5. Unless otherwise indicated, all observations on the leaf should be recorded on sixth leaf from the top on main stem.
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V. Grouping of varieties

  1. The collection of varieties to be grown should be divided into groups to facilitate the assessment of distinctness.  Characteristics which are suitable for grouping purposes are those which do not vary, or to vary only slightly, within a variety. Their various states of expression should be  fairly evenly distributed through out the collection.
      

  2. It is recommended that the competent authorities use the following characteristics for grouping varieties:

      i.       Seedling: premature flowering resistance (C. olitorius varieties only) (characteristic 1)

    ii.       Stem: colour (characteristic 4)

    iii.       Time of flowering (characteristic 9)

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VI. Characteristics and symbols

  1. To assess distinctness, uniformity and stability, the characteristics and their states as given in the table of characteristics should be used.
  2. Notes (1 to 9), for the purpose of electronic data processing are given opposite the states of each characteristic. 
  3. Legend:
      
    (
    *)Characteristics should be used every growing period for the examinations of all varieties and should always be included in the descriptions of the variety, except when the state of expression of a preceding characteristic or regional conditions render this impossible
      
    (+) See explanations on the Table of characteristics in Chapter VIII.

  4. The optimum stage of plant growth for assessment of each characteristic is indicated in the sixth column of Table of characteristic
       
  5. Type of assessment of characteristic indicated in column seven of Table of characteristic is as follows.
       
    MG : Measurement by a single observation of a group of plants or parts of plants 

    MS : Measurement of a number of individual plants or parts of plants

    VG : Visual assessment by a single observation of a group of plants or parts of plants

    VS : Visual assessment by observation of individual plants or parts of plants
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VII. Table of Characteristics

Sl.No.

Characteristics

States

Note

Example varieties

stage of  
observation

Type of assessment

C.olitorius

C.capsularis

1.  
(*)  
(+)

C. olitorius varieties  only:  
Seedling: premature flowering    resistance

 

absent

   

present

1

 

2

JRO 632, JRO 3690, JRO  66

JRO 524, JRO 7835, JRO 878, JRO 8432, JRO   128

 

after 35 days of sowing

 

 

 

VG

2.  
(*)

Leaf : lamina colour

pale green  
green  
red

1
2
3

 

 

after 60 days of sowing (at full foliage stage)

VG

3.  
(*)

Leaf : vein colour

pale green  
green  
red

1
2
3

 

full foliage

VG

4.  
(*)

Stem : colour

pale green  
green  
red  
purple  
coppery red

1
2
3
4
5

   
JRO 524 JRO  66  
JRO  878  

 

 

  JRC  321

full foliage

VG

5.  
(*)

 

Stipule : colour

pale green  
green  
red  
tip red

1
2
3
4

 

full foliage

VG

 

6.
(*)  
(+)

Plant : branching habit

eeak (no effective branching)  
medium (only primary branching)  
strong (secondary branching)

3


5


7

 

 

pre bud

 

VG

7.  
(*)  
(+)

Leaf : angle

erect (0-40o)  
semi-erect (41-80o)  
horizontal (81-120o)  
drooping (121-180o)

1
3

5

7

 

 

pre bud

MS

8.  
(*)  
(+)

Leaf : shape

ovate  
ovate-lanceolate  
lanceolate  
elliptical  
cordate  
palmate

1
2
3
4
5
6

pre bud

VG

9.  
(*)

Time of flowering (50% of the plants with at least one open flower)

early (<81 days)





medium(81-100 days)  
late (>100 days)

3





5

7

 

 

 

   

JRO 632, JRO 66, JRO 524, JRO 7835

Bidhan Pat-1, Bidhan, Pat-2, Bidhan Pat-3

JRC  321

 

VG

10.  
(*)

Flower bud : pigmentation of calyx

Pale green  
green  
red

1
2
3

 

appearance of flower buds

VG

11.
(*)  
(+)

Plant  : height

short ( <301 cm)  
medium (301-400 cm)  
tall (> 400  cm)

3
5

7

 

 

pre-bud stage (before development of first flower)

MS

12.  
(*)

 

 

Time of  harvest maturity

early (<91 days)  
medium(91-110 days)  
late (>110 days)

3
5

7

 


JRO 632, JRO 66, JRO 524, JRO 7835

early pod

VG

13.
(*)

Fruit : pigmentation

pale-green
green  
red

1
2
3

early fruiting (before pod browning)

VS 

14.  
(*)  
(+)

C. olitorius varieties  only :
Pod : dehiscence

absent  
present

1
2

 

 

near maturity

VG

15.  
(*)

 

Seed : size (weight of 1000 seeds at 10% moisture content)

small (< 2 g)  
medium (2-3 g)  
large (> 3g)

3
5
7

 

 

harvest maturity

MG

16.  
(*)

C.olitorius varieties only:
Seed : colour

blue  
steel grey  
chocolate brown
black

1
2
3
4

 

 

 

harvest maturity

VG

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VIII. Explanations on the table of characteristics

 

                       

Characteristic 1. Seedling : premature flowering resistance (C. olitorius varieties only)

 

This characteristics should be recorded after 35 days of sowing.

 

Absent : Variety of tossa jute (C. olitorius) flowers prematurely in seedling stage after one month of sowing, if sown before middle of April.  

Present : Variety of tossa jute (C. olitorius) does not flowers premanturely in seedling stage if sown before of April and flowers at least after 100days

 


Characteristic 6. Plant : branching habit  

3.weak (no effective branching) 5. (only medium primary branches)

7strong(primary and secondary branching)

 

 

 

Characteristic 7. Leaf : angle

This characteristic should be recorded as the angle between the stem and the line connecting the base and the centre of mid-rib of the leaf starting from the 6th leaf from top of the main stem at pre-bud stage (average of 10 leaves)

 

 

1.erect 3. semi erect 5.horizontal 7.droping

Characteristic 8. Leaf : shape

This characteristic should be recorded on leaves (starting from 6th leaf from top of the main stem) at pre-bud stage (average of 10 leaves)  

ovate ovate lanceolate lanceolate elliptical cordate palmate

 

Characteristic 11. Plant : height

This characteristic should be recorded as height of the main stem measured from soil level to the point of forking at pre-bud stage (before development of first flower)  


Characteristic 14. Pod : dehiscence (C. olitorius varieties only)  

 absent present
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VIII. Technical questionnaire

 

Reference Number
(not to be filled in by the applicant)

 

Technical questionnaire  
to be completed in connection with an application for plant breeders' rights  

 

1. Species:                                            Corchorus olitorius L./ Corchorus capsularis L.
                                                                                             
Jute

 

2.      Applicant (Name and address) :  

 

 

 

3.  Proposed denomination or breeder's reference

  

  

4.  Information on origin, maintenance and reproduction of the variety

 

 

 

 

5. Characteristics of the variety to be indicated (the number in brackets refers to the corresponding characteristic in Test Guidelines; please mark the state of expression which best corresponds).  

S.No. Characteristics States Example varieties       Notes

C.olitorius

C.capsularis

5.1
(1)

C. olitorius varieties only:
Seedling : premature flowering resistance

 

absent


present

JRO 632, JRO 3690, JRO  66
JRO 524, JRO 7835, JRO 878, JRO 8432, JRO   128

   

1[  ]


2[  ]

5.2
(4)

Steam: Color 

Pale green
green
red
purple
coppery red


JRO 524 
JRO  66
JRO  878





JRC 321

1 [ ]
2 [ ]
3 [ ]
4 [ ]
5 [ ]

5.3
(9)
Time of  flowering (50% of the plants with at least one open flower)

early (<81 days)

 

 
medium (81-100 days)
late (>100 days








JRO 632, JRO 66, 
JRO 524, JRO 7835

Bidhan Pat-1, Bidhan, Pat-2, Bidhan Pat-3

JRC321

3[  ]



5[  ]

7[  ]

6. Similar varieties and differences from these varieties  

Denomination of
Similar variety

Characteristic in
which the similar variety is different 0)

State of expression of similar Variety

State of expression of candidate variety
               
0)  In the case of identical states of expressions of both varieties, please indicate the size of the difference.  

7. Additional information which may help to distinguish the variety

 

      7.1 Resistance to pests and diseases

 

      7.2 Special conditions for the examination of the variety

 

           7.3 Other information

 

8.  Authorization for release

 

(a)   Does the variety require prior authorization for release under legislation concerning the protection of the environment, human and animal health?

 

                     Yes              {     }                                                         No         {      }

 

(b)    Has such authorization been obtained?

 

                      Yes             {     }                                                         No        {      }     


If the answer to that question is ‘Yes’, please attach a copy of such an authorization   

 

[End of the document]

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