Chickpea

The Plant Variety Protection (PVP) Authority decides when, where and in what quantity and quality the plant material required for testing the variety is to be delivered. Applicants submitting material from a country other than India must make sure that all customs formalities are complied with. The minimum quantity of seed to be supplied by the applicant should be: 2000 grams (in one submission only)
The seed should meet the minimum requirements for germination capacity, moisture content and physical purity prescribed for certified seed in India. Especially for storage, which requires a higher standard, the applicant should state, the actual germination capacity which should be as high as possible. The seed supplied should be visibly healthy, not lacking in vigour or affected by any important pest or disease.
The plant material must not have undergone any treatment unless the competent authorities allow or request such treatment. If it has been treated, full details of the treatment must be given.

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III. Conduct of tests

The minimum duration of tests should normally be two independent similar growing seasons with reference to the ecosystem of the variety submitted for DUS test.
The test should normally be conducted at two test locations. If any important characteristic of the variety can not be seen at these places, the variety may be tested at an additional place.

The test should be carried out under conditions ensuring normal growth. The size of the plot should be such that plants or parts of plant may be removed for measuring and counting without prejudice to the observation which must be made upto the end of the growing period. Each test should include a minimum of 450 plants which should be divided among 3 replications. Separate plots for observation and for measuring can only be used if they have been subjected to similar environmental conditions.

Test plot design

No. of rows	: 6
Row length	: 5 m
Row to row distance	: 45 cm
Plant to plant distance	: 20 cm
Number of replications	: 3

Observations should not be recorded on plants in border rows.
Additional tests for special purpose may be established.

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IV. Methods and observations

The characteristics described in the Table of characteristics should be used for the testing of varieties for DUS .
For the assessment of distinctness and stability, observations should be made on 30 plants or parts of 30 plants which should be divided among 3 replications (10 plants in each replication).
For the assessment of uniformity of characteristics on the plot as a whole (visual assessment by a single observation of a group of plants or parts of plants), a population standard of 1% with an acceptance probability of at least 95% should be applied. In the case of a sample size of 100 plants, the number of off-types should not exceed 3.
For the assessment of colour characteristics, it is recommended that Royal Horticultural Society (RHS) colour chart should be used..

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V.Grouping of varieties

The collection of varieties to be grown should be divided into groups to facilitate the assessment of distinctness. Characteristics, which are suitable for grouping purposes, are those, which are known from experience not to vary, or to vary only slightly, within a variety. Their various states of expression should be fairly evenly distributed throughout the collection.
It is recommended that the competent authorities use the following characteristics for grouping varieties:

                                                    i.        Time of flowering (characteristic 3)

                                                  ii.        Seed: colour (characteristic 13 )

                                                 iii.        Seed : size (characteristic 14 )

                                                iv.        Seed: type (characteristic 18 )

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VI. Characteristics and symbols

To assess distinctness, uniformity and stability, the characteristics and their states as given in the Table of characteristics should be used.
Notes (1 to 9), for the purposes of electronic data processing, are given opposite the states of each characteristic.
Legend :

(*) Characteristics that should be used every growing period for the examinations of all varieties and should always be included in the description of the variety, except when the state of expression of a preceding characteristic or regional environmental conditions render this impossible.

(+) See explanations on the Table of characteristics in Chapter VII.
The optimum stage of plant growth for assessment of each characteristic is indicated in the sixth column of Table of characteristics.
Type of assessment of characteristics indicated in column seven of Table of characteristics is as follows :

MG : Measurement by a single observation of a group of plants or parts of plants

MS : Measurement of a number of individual plants or parts of plants

VG : Visual assessment by a single observation of a group of plants or parts of plants

VS : Visual assessment by observation of individual plants or parts of plants

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VII. Table of characteristics

S.No	Characteristics	States	Note	Example Varieties	Stage of observation	Type of assessment
1. (*)	Stem: anthocyanin colouration	absent present	1 9		before flowering	VS
2.	Stem: height of insertion of first flower	low(<8 nodes) medium(8-15 nodes) high(>15 nodes)	3 5 7		insertion of first flower	MS
3. (*)	Time of flowering (50% of the plants with at least one open flower)	early (<60 days) medium(60-80 days) late(>80 days)	3 5 7			VG
4.	Plant: number of flowers per peduncle	single twin multiple	1 2 3		50% flowering	VS
5. (*) (+)	Plant: growth habit	erect(0-15⁰ from vertical) semi-erect(16-60⁰ from vertical) spreading(61-80⁰ from vertical)	3 5 7		flowering	VG
6. (*)	Plant: green color intensity of foliage	light medium dark	3 5 7		flowering	VG
7. (*)	Leaflet : size (middle of the plant and middle of the leaf)	small(<10mm long) medium(10-15mm long) large(>15mm long)	3 5 7		flowering	MS
8. (*)	Flower: colour	white pink blue	1 2 3		flowering	VG
9.	Flower : stripes on standard	absent present	1 9		flowering	VG
10.	Peduncle: length	short medium long	3 5 7		pod development	MS
11 (*)	Plant : height	short(<45 cm) medium(45-60 cm) tall(>60 cm)	3 5 7		fully developed green pod	MS
12. (*)	Pod: size	small(<15 mm long) medium(15-20 mm long) large(>20 mm long)	3 5 7		harvest maturity	MS
13. (*)	Seed: colour	beige(kabuli) brown beige green yellow pink brown dark brown	1 2 3 4 5 6 7		one month of harvest	VG
14. (*)	Seed : size(weight of 100 seeds at 10% moisture content)	desi types small(<15g) medium(15-22g) large(23-30g) very large(>30g) kabuli types small(<25g) medium(25-30g) large(31-40g) very large(>40g)	3 5 7 9 3 5 7 9		one month of harvest	MG
15. (*) (+)	Seed: shape	pea-shaped owl’s head angular	1 2 3		one month of harvest	VG
16. (*) (+)	Seed: testa texture	rough smooth tuberculated	1 2 3		one month of harvest	VG
17. (*)	Seed : ribbing	absent present	1 9		one month of harvest	VG
18	Seed: type	desi kabuli	1 2		one month of harvest	VG

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VIII. Explanations on the table of characteristics

Characteristic 5. Plant : growth habit


3.erect	5.semi erect	7.spreading

Characteristic 15. Seed : shape


1.pea shaped	2.owl’s head	3.angular

Characteristic 16. Seed : testa texture


rough	smooth	tuberculated

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IX. Technical questionnaire

				Reference Number (not to be filled in by the applicant)

Technical questionnaire to be completed in connection with an application for plant breeders' rights
1. Species: (Cicer arietinum L.) Chickpea
2. Applicant (Name and address) :
3. Proposed denomination or breeder's reference
4. Information on origin, maintenance and reproduction of the variety 4.1 Generic origin and breeding methods 4.2 Other informations
5. Characteristics of the variety to be indicated (the number in brackets refers to the corresponding characteristic in Test Guidelines; please mark the state of expression which best corresponds).
S.No.	Characteristics		States		Example varieties		Notes
1 (3)	Time of flowering (50% of the plants with at least one open flower)		early (<60 days) medium (60-80 days) late (>80 days)				3 [ ] 5 [ ] 7 [ ]
2 (13)	Seed: Color		beige (kabuli) brown beige green yellow pink brown dark brown				1 [ ] 2 [ ] 3 [ ] 4 [ ] 5 [ ] 6 [ ] 7 [ ]
3 (14)	Seed : size(weight of 100 seeds at 10% moisture content)		desi types small(<15g) medium(15-22g) large(23-30g) very large(>30g) kabuli types small(<25g) medium(25-30g) large(31-40g) very large(>40g)				3[ ] 5[ ] 7[ ] 9[ ] 3[ ] 5[ ] 7[ ] 9[ ]
4. (18)	Seed: Type		Desi Kabuli				1[ ] 2[ ]
6. Similar varieties and differences from these varieties
Denomination of Similar variety		Characteristic in which the similar variety is different ⁰⁾		State of expression of similar Variety		State of expression of candidate variety

⁰⁾In the case of identical states of expressions of both varieties, please indicate the size of the difference.
7. Additional information which may help to distinguish the variety 7.1 Resistance to pests and diseases 7.2 Special conditions for the examination of the variety 7.3 Other information
8. Authorization for release (a) Does the variety require prior authorization for release under legislation concerning the protection of the environment, human and animal health? Yes { } No { } (b) Has such authorization been obtained? Yes { } No { } If the answer to that question is ‘Yes’, please attach a copy of such an authorization

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